Anti-Actin Antibody

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Anti-Actin Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-Actin Antibody
Validated applications
WB,IHC-P
Species reactivity
Reacts with: Human, Mouse, Rat
Specificity
Human Actin
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human Actin
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human Actin, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Actin Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:1000
IHC-P 1:500-1:2000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Actin Antibody (Rabbit Polyclonal antibody) Images

Anti-ACTA1 rabbit polyclonal antibody at 1:500 dilution

Lane A: Hela Whole Cell Lysate

Lane B: A549 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit  IgG (H&L) /Dylight 800  at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:42 kDa

Observed band size:45 kDa

Immunochemical staining ACTA1 in human prostate with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Immunochemical staining ACTA1 in human skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Immunochemical staining ACTA1 in mouse skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Immunochemical staining ACTA1 in rat skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).

Anti-Actin Antibody: Alternative Names

Anti-ACTA Antibody; Anti-ASMA Antibody; Anti-CFTD Antibody; Anti-CFTD1 Antibody; Anti-CFTDM Antibody; Anti-MPFD Antibody; Anti-NEM1 Antibody; Anti-NEM2 Antibody; Anti-NEM3 Antibody

Actin Background Information

Nemaline myopathy (NM) is one of the most common congenital nondystrophic myopathies and is characterized by muscle weakness, often from birth. Mutations in ACTA1 are a frequent cause of NM (ie, NEM3). ACTA1 encodes alpha-actin 1, the main constituent of the sarcomeric thin filament. The mechanisms by which mutations in ACTA1 contribute to muscle weakness in NEM3 are incompletely understood. Mutations in skeletal muscle α-actin 1-encoding gene (ACTA1) cause autosomal dominant or recessive myopathies with marked clinical and pathological heterogeneity. Patients typically develop generalized or limb-girdle pattern of weakness, that clinically severe ACTA1-related myopathy can present with muscle morphological findings suggestive of cytoplasmic body myopathy in the absence of definite nemaline rods. The Asn94Lys mutation in skeletal muscle sarcomeric α-actin may be linked to this histological appearance. 
Full Name
actin, alpha 1, skeletal muscle

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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