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Rat CYP4F1 clonación del ADN o clonación génica(Vector de expresión)

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Rat CYP4F1 Información de producto de clon de cDNA
Gene_bank_ref_id:NM_019623.2
Tamaño de cDNA:1575bp
Descripción de cDNA:Full length Clone DNA of Rattus norvegicus cytochrome P450, family 4, subfamily f, polypeptide 1.
Sinónimo de gen:Cyp4f2, Cyp4f14
Especie:Rat
Vector:pUC19 Vector
Plasmid:cpUC19-ratCyp4f1
Sitio de restricción:
Secuencia de etiquetas:
Descripción de la secuencia:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 73T>C(F25L), 1116G>A(W372X), 1145C>T(T382I) and 1156A>C(K386Q).
Sequencing primers:M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Almacenamiento:The lyophilized plasmid can be stored at room temperature for three months.
Rat CYP4F1 Gene Plasmid Map
Rat CYP4F1 Gene cDNA Clone (full-length ORF Clone)
pUC19 vector Vector Information:

pUC19 is a small, high-copy number E. coli plasmid cloning vector, of which multiple cloning sites as shown below. The molecule is a small double-stranded circle, 2686 base pairs in length. pUC19 encodes the N-terminal fragment of b-galactosidase (lacZa), which allows for blue/white colony screening (i.e., a-complementation), as well as a pUC origin of replication.

pUC19 vector Usage Suggestion:

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
Rat CYP4F1 clonación del ADN o clonación génica(Vector de expresión) on other vectors
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  • Rat CYP4F1 Gene cDNA Clone (full-length ORF Clone)
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