Pedido rápido

Canino IL18/IL-18/Interleukin 18 clonación del ADN o clonación génica(vector de clonación), C-Flag Etiqueta

    Hoja de datosReseñasProductos relacionadosProtocolos
    Canino IL18 Información de producto de clon de cDNA
    Gene_bank_ref_id:NM_001003169.1
    Tamaño de cDNA:582bp
    Descripción de cDNA:Full length Clone DNA of Canis lupus familiaris interleukin 18 (interferon-gamma-inducing factor) with C terminal Flag tag.
    Sinónimo de gen:IGIF, IL18
    Especie:Canine
    Vector:pCMV3-C-FLAG
    Plasmid:
    Sitio de restricción:
    Secuencia de etiquetas:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
    Descripción de la secuencia:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    Shipping_carrier:Each tube contains lyophilized plasmid.
    Almacenamiento:The lyophilized plasmid can be stored at room temperature for three months.
    FLAG Tag Info

    FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

    A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

    The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

    Canino IL18/IL-18/Interleukin 18 clonación del ADN o clonación génica(vector de clonación), C-Flag Etiqueta on other vectors
    Canino IL18/IL-18/Interleukin 18 clonación del ADN o clonación génica(vector de clonación), C-GFPSpark EtiquetaDG70020-ACG$225
    Canino IL18/IL-18/Interleukin 18 clonación del ADN o clonación génica(vector de clonación), C-OFPSpark EtiquetaDG70020-ACR$225
    Canino IL18/IL-18/Interleukin 18 clonación del ADN o clonación génica(vector de clonación), C-Flag EtiquetaDG70020-CF$195
    Canino IL18/IL-18/Interleukin 18 clonación del ADN o clonación génica(vector de clonación), C-His EtiquetaDG70020-CH$195
    Canino IL18/IL-18/Interleukin 18 clonación del ADN o clonación génica(vector de clonación), C-Myc EtiquetaDG70020-CM$195
    Canino IL18/IL-18/Interleukin 18 clonación del ADN o clonación génica(vector de clonación), C-HA EtiquetaDG70020-CY$195
    Canino IL18/IL-18/Interleukin 18 clonación del ADN o clonación génica(Vector de expresión)DG70020-G$75
    Canino IL18/IL-18/Interleukin 18 clonación del ADN o clonación génica(vector de clonación), N-Flag EtiquetaDG70020-NF$195
    Canino IL18/IL-18/Interleukin 18 clonación del ADN o clonación génica(vector de clonación), N-His EtiquetaDG70020-NH$195
    Canino IL18/IL-18/Interleukin 18 clonación del ADN o clonación génica(vector de clonación), N-Myc EtiquetaDG70020-NM$195
    Canino IL18/IL-18/Interleukin 18 clonación del ADN o clonación génica(vector de clonación), N-HA EtiquetaDG70020-NY$195
    Canino IL18/IL-18/Interleukin 18 clonación del ADN o clonación génica(vector de clonación)DG70020-UT$195
     Más información sobre los vectores de expresión
    Product nameProduct name
    Fondo

    Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1−like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cells-, IL-4− and STAT6−dependent fashion. IL-18 and T cell receptor−mediated stimulation could induce naïve CD4+ T cells to develop into IL-4−producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-α and IL-1β, suggesting that monokine expression can feed back to promote Th1 cell development in synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA.

    Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

    Referencias
  • Dinarello CA. (1999) IL-18: A TH1-inducing, proinflammatory cytokine and new member of the IL-1 family. J Allergy Clin Immunol. 103: 11-24.
  • Takeda K, et al.. (1998) Defective NK cell activity and Th1 response in IL-18-deficient mice. Immunity. 8(3): 383-90.
  • Gracie JA, et al.. (1999) A proinflammatory role for IL-18 in rheumatoid arthritis. J Clin Invest. 104(10): 1393-401.
  • Size / Price
    Catálogo: DG70020-CF
    Precio de lista: 
    Precio:      (You Save: )
    Tamaño:
    Cantidad:+-
    Disponibilidad
    Añadir a carroBulk Discount Requiry

    Datasheet & Documentation

    Contact Us
    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    Nota: Todos los productos son "PARA SU USO ÚNICAMENTE EN INVESTIGACIÓN Y NO INDICADOS PARA SU USO DIAGNÓSTICO O TERAPÉUTICO"