Anti-CA5A Antibody (Rabbit Polyclonal antibody) General Information
Reacts with: Human
Recombinant Human Carbonic Anhydrase VA / CA5A protein (Catalog#10500-H08E)
Produced in rabbits immunized with purified, recombinant Human Carbonic Anhydrase VA / CA5A (rh Carbonic Anhydrase VA / CA5A; Catalog#10500-H08E; NP_001730.1; Ala 40-Ser 305). Carbonic Anhydrase VA / CA5A specific IgG was purified by Human Carbonic Anhydrase VA / CA5A affinity chromatography.
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CA5A Antibody (Rabbit Polyclonal antibody) Validated Applications
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-CA5A Antibody (Rabbit Polyclonal antibody) Images
Anti-CA5A rabbit polyclonal antibody at 1:500 dilution
Lane A: NCI-H460 Whole Cell Lysate
Lane B: A549 Whole Cell Lysate
Lysates/proteins at 60 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
Predicted band size:35 kDa
Observed band size:35 kDa
(We are unsure as to the identity of these extra bands.)
Anti-CA5A Antibody Alternative Names
Anti-CA5 Antibody;Anti-CA5AD Antibody;Anti-CA5D Antibody;Anti-Carbonic Anhydrase VA Antibody;Anti-CAV Antibody;Anti-CAVA Antibody;Anti-GS1-21A4.1 Antibody
CA5A Background Information
Carbonic anhydrase 5A, mitochondrial, also known as Carbonate dehydratase VA, Carbonic anhydrase VA, CA-VA and CA5A, is a member of the alpha-carbonic anhydrase family. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA5A / CA-VA is activated by histamine, L-adrenaline, L- and D-histidine, and L- and D-phenylalanine. It is inhibited by coumarins, sulfonamide derivatives such as acetazolamide and Foscarnet (phosphonoformate trisodium salt).
carbonic anhydrase VA, mitochondrial
Strausberg, R.L. et al., 2002, Proc. Natl. Acad. Sci. USA 99:16899 - 903. Liao, S.Y. et al., 2003, J. Med. Genet. 40:257 - 262. Temperini C.et al., 2006, Chemistry 12: 7057-66. Temperini C.et al., 2006, J. Med. Chem. 49: 3019-27. Supuran, C. T. et al., 2008, Curr Pharm Des. 14 (7): 601-602. Elleuche, S. et al., 2009, Curr Genet. 55 (2): 211-222.