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Cangrejero GAPDH clonación del ADN o clonación génica(vector de clonación), N-Flag Etiqueta

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    Cangrejero GAPDH Información de producto de clon de cDNA
    Gene_bank_ref_id:XM_005569912.1
    Tamaño de cDNA:1008bp
    Descripción de cDNA:Full length Clone DNA of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) glyceraldehyde-3-phosphate dehydrogenase with N terminal Flag tag.
    Sinónimo de gen:GAPDH
    Especie:Cynomolgus
    Vector:pCMV3-N-FLAG
    Plasmid:
    Sitio de restricción:
    Secuencia de etiquetas:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
    Descripción de la secuencia:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    Shipping_carrier:Each tube contains lyophilized plasmid.
    Almacenamiento:The lyophilized plasmid can be stored at room temperature for three months.
    FLAG Tag Info

    FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

    A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

    The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

    Cangrejero GAPDH clonación del ADN o clonación génica(vector de clonación), N-Flag Etiqueta on other vectors
    Cangrejero GAPDH clonación del ADN o clonación génica(vector de clonación), C-GFPSpark EtiquetaCG90448-ACG$225
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    Cangrejero GAPDH clonación del ADN o clonación génica(vector de clonación), N-OFPSpark EtiquetaCG90448-ANR$225
    Cangrejero GAPDH clonación del ADN o clonación génica(vector de clonación), C-Flag EtiquetaCG90448-CF$195
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    Cangrejero GAPDH clonación del ADN o clonación génica(vector de clonación), C-Myc EtiquetaCG90448-CM$195
    Cangrejero GAPDH clonación del ADN o clonación génica(vector de clonación), C-HA EtiquetaCG90448-CY$195
    Cangrejero GAPDH clonación del ADN o clonación génica(Vector de expresión)CG90448-G$75
    Cangrejero GAPDH clonación del ADN o clonación génica(vector de clonación), N-Flag EtiquetaCG90448-NF$195
    Cangrejero GAPDH clonación del ADN o clonación génica(vector de clonación), N-His EtiquetaCG90448-NH$195
    Cangrejero GAPDH clonación del ADN o clonación génica(vector de clonación), N-Myc EtiquetaCG90448-NM$195
    Cangrejero GAPDH clonación del ADN o clonación génica(vector de clonación), N-HA EtiquetaCG90448-NY$195
    Cangrejero GAPDH clonación del ADN o clonación génica(vector de clonación)CG90448-UT$195
     Más información sobre los vectores de expresión
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    Fondo

    Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions.

    Referencias
  • Hara MR, et al. (2006) Neuroprotection by pharmacologic blockade of the GAPDH death cascade. PNA. 103 (10): 3887-9.
  • Hara MR, et al. (2006) GAPDH as a sensor of NO stress.Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1762 (5): 502-9.
  • Tarze A, et al. (2007) GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilizationGAPDH and apoptosis. Oncogene. 26: 2606-20.
  • Yi MK, et al. (2000) Functional Significance of the Interaction of Hepatitis A Virus RNA with Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH): Opposing Effects of GAPDH and Polypyrimidine Tract Binding Protein on Internal Ribosome Entry Site Function. Journal of Virology. 74 (14) : 6459-68.
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    Catálogo: CG90448-NF
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