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Humano PSMA/FOLH1/GCPII transcript variant 2 clonación del ADN o clonación génica(Vector de expresión)

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    Humano FOLH1 Información de producto de clon de cDNA
    Gene_bank_ref_id:NM_001014986.1
    Tamaño de cDNA:2160bp
    Descripción de cDNA:Full length Clone DNA of Homo sapiens folate hydrolase (prostate-specific membrane antigen) 1.
    Sinónimo de gen:PSM, FGCP, FOLH, GCP2, PSMA, mGCP, GCPII, NAALAD1, NAALAdase
    Especie:Human
    Vector:pGEM-T Vector
    Plasmid:pGEM-FOLH1-transcriptvariant2
    Sitio de restricción:
    Secuencia de etiquetas:
    Descripción de la secuencia:Identical with the Gene Bank Ref. ID sequence except for the point mutation 843 T/C not causing the amino acid variation.
    Sequencing primers:SP6 and T7 or M13-47 and RV-M
    ( We provide with FOLH1 qPCR primers for gene expression analysis, HP104466 )
    Promoter:
    Application:
    Antibiotic in E.coli:Ampicillin
    Antibiotic in mammalian cell:
    Shipping_carrier:Each tube contains lyophilized plasmid.
    Almacenamiento:The lyophilized plasmid can be stored at room temperature for three months.
    pGEM-T Vector Information

    The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

    pGEM-T Simple Usage Suggestion:

    The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

    Vector Sequence Download
    Humano PSMA/FOLH1/GCPII transcript variant 2 clonación del ADN o clonación génica(Vector de expresión) on other vectors
    Humano PSMA/FOLH1/GCPII transcript variant 2 clonación del ADN o clonación génica(vector de clonación), C-GFPSpark EtiquetaHG15876-ACG$245
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    Humano PSMA/FOLH1/GCPII transcript variant 2 clonación del ADN o clonación génica(vector de clonación), C-HA EtiquetaHG15876-CY$215
    Humano PSMA/FOLH1/GCPII transcript variant 2 clonación del ADN o clonación génica(vector de clonación), N-Flag EtiquetaHG15876-NF$215
    Humano PSMA/FOLH1/GCPII transcript variant 2 clonación del ADN o clonación génica(vector de clonación), N-His EtiquetaHG15876-NH$215
    Humano PSMA/FOLH1/GCPII transcript variant 2 clonación del ADN o clonación génica(vector de clonación), N-Myc EtiquetaHG15876-NM$215
    Humano PSMA/FOLH1/GCPII transcript variant 2 clonación del ADN o clonación génica(vector de clonación), N-HA EtiquetaHG15876-NY$215
    Humano PSMA/FOLH1/GCPII transcript variant 2 clonación del ADN o clonación génica(vector de clonación)HG15876-UT$215
     Más información sobre los vectores de expresión
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    Fondo

    Glutamate carboxypeptidase 2, also known as Glutamate carboxypeptidase II, Membrane glutamate carboxypeptidase, Prostate-specific membrane antigen, GCPII, PSMA, FOLH1, and NAALAD1, is a single-pass type I I membrane protein which belongs to the peptidase M28 family and M28B subfamily. FOLH1 is highly expressed in prostate epithelium. It is detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon, brain (at protein level), and the capillary endothelium of a variety of tumors. FOLH1 has both folate hydrolase and N-acetylated alpha linked acidic dipeptidase (NAALADase) activity. It has a preference for tri-alpha-glutamate peptides. Genetic variation in FOLH1 may be associated with low folate levels and consequent hyperhomocysteinemia. This condition can result in increased risk of cardiovascular disease, neural tube defects, and cognitive deficits. FOLH1 also shows a promising role in directed imaging and therapy of recurrent or metastatic disease.

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