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Cangrejero GAPDH clonación del ADN o clonación génica(Vector de expresión)

Hoja de datosReseñasProductos relacionadosProtocolos
Cynomolgus GAPDH Información de producto de clon de cDNA
Gene_bank_ref_id:XM_005569912.1
Tamaño de cDNA:1008bp
Descripción de cDNA:Full length Clone DNA of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) glyceraldehyde-3-phosphate dehydrogenase.
Sinónimo de gen:GAPDH
Especie:Cynomolgus
Vector:pGEM-T Vector
Plasmid:pGEM-cynoGAPDH
Sitio de restricción:
Secuencia de etiquetas:
Descripción de la secuencia:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 282T/C, 300T/C, 912C/T not causing the amino acid variation. Please check the sequence information before order.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Almacenamiento:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
Cangrejero GAPDH clonación del ADN o clonación génica(Vector de expresión) on other vectors
Cangrejero GAPDH clonación del ADN o clonación génica(vector de clonación), C-GFPSpark EtiquetaCG90448-ACG$225
Cangrejero GAPDH clonación del ADN o clonación génica(vector de clonación), C-OFPSpark EtiquetaCG90448-ACR$225
Cangrejero GAPDH clonación del ADN o clonación génica(vector de clonación), N-GFPSpark EtiquetaCG90448-ANG$225
Cangrejero GAPDH clonación del ADN o clonación génica(vector de clonación), N-OFPSpark EtiquetaCG90448-ANR$225
Cangrejero GAPDH clonación del ADN o clonación génica(vector de clonación), C-Flag EtiquetaCG90448-CF$195
Cangrejero GAPDH clonación del ADN o clonación génica(vector de clonación), C-His EtiquetaCG90448-CH$195
Cangrejero GAPDH clonación del ADN o clonación génica(vector de clonación), C-Myc EtiquetaCG90448-CM$195
Cangrejero GAPDH clonación del ADN o clonación génica(vector de clonación), C-HA EtiquetaCG90448-CY$195
Cangrejero GAPDH clonación del ADN o clonación génica(Vector de expresión)CG90448-G$75
Cangrejero GAPDH clonación del ADN o clonación génica(vector de clonación), N-Flag EtiquetaCG90448-NF$195
Cangrejero GAPDH clonación del ADN o clonación génica(vector de clonación), N-His EtiquetaCG90448-NH$195
Cangrejero GAPDH clonación del ADN o clonación génica(vector de clonación), N-Myc EtiquetaCG90448-NM$195
Cangrejero GAPDH clonación del ADN o clonación génica(vector de clonación), N-HA EtiquetaCG90448-NY$195
Cangrejero GAPDH clonación del ADN o clonación génica(vector de clonación)CG90448-UT$195
 Más información sobre los vectores de expresión
Product nameProduct name
Fondo

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions.

Referencias
  • Hara MR, et al. (2006) Neuroprotection by pharmacologic blockade of the GAPDH death cascade. PNA. 103 (10): 3887-9.
  • Hara MR, et al. (2006) GAPDH as a sensor of NO stress.Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1762 (5): 502-9.
  • Tarze A, et al. (2007) GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilizationGAPDH and apoptosis. Oncogene. 26: 2606-20.
  • Yi MK, et al. (2000) Functional Significance of the Interaction of Hepatitis A Virus RNA with Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH): Opposing Effects of GAPDH and Polypyrimidine Tract Binding Protein on Internal Ribosome Entry Site Function. Journal of Virology. 74 (14) : 6459-68.
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