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Humano Carbonic Anhydrase III clonación del ADN o clonación génica(vector de clonación), C-HA Etiqueta

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Human CA3 Información de producto de clon de cDNA
Gene_bank_ref_id:NM_005181.3
Tamaño de cDNA:783bp
Descripción de cDNA:Full length Clone DNA of Homo sapiens carbonic anhydrase III, muscle specific with C terminal HA tag.
Sinónimo de gen:Car3, CAIII
Especie:Human
Vector:pCMV3-C-HA
Plasmid:
Sitio de restricción:
Secuencia de etiquetas:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
Descripción de la secuencia:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Almacenamiento:The lyophilized plasmid can be stored at room temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

Humano Carbonic Anhydrase III clonación del ADN o clonación génica(vector de clonación), C-HA Etiqueta on other vectors
Humano Carbonic Anhydrase III clonación del ADN o clonación génica(vector de clonación), C-GFPSpark EtiquetaHG10503-ACG$225
Humano Carbonic Anhydrase III clonación del ADN o clonación génica(vector de clonación), C-OFPSpark EtiquetaHG10503-ACR$225
Humano Carbonic Anhydrase III clonación del ADN o clonación génica(vector de clonación), N-GFPSpark EtiquetaHG10503-ANG$225
Humano Carbonic Anhydrase III clonación del ADN o clonación génica(vector de clonación), N-OFPSpark EtiquetaHG10503-ANR$225
Humano Carbonic Anhydrase III clonación del ADN o clonación génica(vector de clonación), C-Flag EtiquetaHG10503-CF$195
Humano Carbonic Anhydrase III clonación del ADN o clonación génica(vector de clonación), C-His EtiquetaHG10503-CH$195
Humano Carbonic Anhydrase III clonación del ADN o clonación génica(vector de clonación), C-Myc EtiquetaHG10503-CM$195
Humano Carbonic Anhydrase III clonación del ADN o clonación génica(vector de clonación), C-HA EtiquetaHG10503-CY$195
Humano Carbonic Anhydrase III clonación del ADN o clonación génica(Vector de expresión)HG10503-M$75
Humano Carbonic Anhydrase III clonación del ADN o clonación génica(vector de clonación), N-Flag EtiquetaHG10503-NF$195
Humano Carbonic Anhydrase III clonación del ADN o clonación génica(vector de clonación), N-His EtiquetaHG10503-NH$195
Humano Carbonic Anhydrase III clonación del ADN o clonación génica(vector de clonación), N-Myc EtiquetaHG10503-NM$195
Humano Carbonic Anhydrase III clonación del ADN o clonación génica(vector de clonación), N-HA EtiquetaHG10503-NY$195
Humano Carbonic Anhydrase III clonación del ADN o clonación génica(vector de clonación)HG10503-UT$195
 Más información sobre los vectores de expresión
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Fondo

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Carbonic anhydrases (CAs) form a family of enzymes that catalyze the rapid conversion of carbon dioxide and water to bicarbonate and protons, a reaction that occurs rather slowly in the absence of a catalyst. The active site of most carbonic anhydrases contains a zinc ion, they are therefore classified as metalloenzymes. Several forms of carbonic anhydrase occur in nature. The primary function of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. Plants contain a different form called β-carbonic anhydrase, which, from an evolutionary standpoint, is a distinct enzyme, but participates in the same reaction and also uses a zinc ion in its active site.

Carbonic anhydrase 3, also known as Carbonate dehydratase III, CA-III and CA3, is a cytoplasm protein which belongs to the alpha-carbonic anhydrase family. CA3 is activated by proton donors such as imidazole and the dipeptide histidylhistidine. It is inhibited by coumarins and sulfonamide derivatives such as acetazolamide. At 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord.

Referencias
  • Ivanov, S.V. et al., 1998, Proc. Natl. Acad. Sci. USA 95:12596-601.
  • Sowden J. et al., 1998, Gene 214:157- 65.
  • Strausberg, R.L. et al., 2002, Proc. Natl. Acad. Sci. USA 99:16899-903.
  • Liao, S.Y. et al., 2003, J. Med. Genet. 40: 257-62.
  • Bataller,L. et al., 2004, Ann Neurol  56 (4): 575-9.
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