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Humano CCL8/MCP-2 clonación del ADN o clonación génica(vector de clonación), C-Flag Etiqueta

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Human CCL8 Información de producto de clon de cDNA
Gene_bank_ref_id:NM_005623.2
Tamaño de cDNA:300bp
Descripción de cDNA:Full length Clone DNA of Homo sapiens chemokine (C-C motif) ligand 8 with C terminal Flag tag.
Sinónimo de gen:HC14, MCP2, MCP-2, SCYA8, SCYA10
Especie:Human
Vector:pCMV3-C-FLAG
Plasmid:
Sitio de restricción:
Secuencia de etiquetas:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Descripción de la secuencia:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Almacenamiento:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Humano CCL8/MCP-2 clonación del ADN o clonación génica(vector de clonación), C-Flag Etiqueta on other vectors
Humano CCL8/MCP-2 clonación del ADN o clonación génica(vector de clonación), C-GFPSpark EtiquetaHG10989-ACG$225
Humano CCL8/MCP-2 clonación del ADN o clonación génica(vector de clonación), C-OFPSpark EtiquetaHG10989-ACR$225
Humano CCL8/MCP-2 clonación del ADN o clonación génica(vector de clonación), C-Flag EtiquetaHG10989-CF$195
Humano CCL8/MCP-2 clonación del ADN o clonación génica(vector de clonación), C-His EtiquetaHG10989-CH$195
Humano CCL8/MCP-2 clonación del ADN o clonación génica(vector de clonación), C-Myc EtiquetaHG10989-CM$195
Humano CCL8/MCP-2 clonación del ADN o clonación génica(vector de clonación), C-HA EtiquetaHG10989-CY$195
Humano CCL8/MCP-2 clonación del ADN o clonación génica(Vector de expresión)HG10989-M$75
Humano CCL8/MCP-2 clonación del ADN o clonación génica(vector de clonación), C-Flag EtiquetaHG10989-M-F$195
Humano CCL8/MCP-2 clonación del ADN o clonación génica(vector de clonación)HG10989-M-N$195
Humano CCL8/MCP-2 clonación del ADN o clonación génica(vector de clonación), N-Flag EtiquetaHG10989-NF$195
Humano CCL8/MCP-2 clonación del ADN o clonación génica(vector de clonación), N-His EtiquetaHG10989-NH$195
Humano CCL8/MCP-2 clonación del ADN o clonación génica(vector de clonación), N-Myc EtiquetaHG10989-NM$195
Humano CCL8/MCP-2 clonación del ADN o clonación génica(vector de clonación), N-HA EtiquetaHG10989-NY$195
Humano CCL8/MCP-2 clonación del ADN o clonación génica(vector de clonación)HG10989-UT$195
 Más información sobre los vectores de expresión
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Fondo

Chemokines are a family of small chemotactic cytokines, or proteins secreted by cells. Chemokines share the same structure similarities such as small size, and the presence of four cysteine residues in conserved locations in order to form their 3-dimensional shape. Some of the chemokines are considered pro-inflammatory which can be induced to recruit cells of the immune system to a site of infection during an immune response, while others are considered homeostatic and are implied in controlling the migration of cells during normal processes of tissue maintenance and development. There are four members of the chemokine family: C-C kemokines, C kemokines, CXC kemokines and CX3C kemokines. The C-C kemokines have two cysteines nearby the amino terminus. There have been at least 27 distinct members of this subgroup reported for mammals, called C-C chemokine ligands-1 to 28. Chemokine ligand 8 (CCL8), also known as monocyte chemoattractant protein 2 (MCP-2), is a small cytokine belonging to the C-C chemokine family. CCL8 functions to activate different immune cells, including mast cells, eosinophils and basophils which are involved in allergic responses, monocytes, and T cells and NK cells which are involved in the inflammatory response. CCL8's ability achieves by binding to different cell surface receptors termed chemokine receptors including CCR1, CCR2B and CCR5. It has been reported that CCL8 is a potent inhibitor of HIV-1 by virtue of its binding to CCR5 which is one of the major co-receptors for HIV-1.

Referencias
  • Laing KJ, et al. (2004) Chemokines. Developmental and comparative immunology. 28 (5): 443-60.
  • Cocchi F, et al. (1995) Identification of RANTES, MIP-1a, and MIP-1b as the major HIV-suppressive factor produced by CD8+ T cells. Science. 270 (5243): 1811–5.
  • Hori T, et al. (2008) CCL8 is a potential molecular candidate for the diagnosis of graft-versus-host disease. Blood. 111 (8): 4403-12.
  • Biber K, et al. (2003) Expression of L-CCR in HEK 293 cells reveals functional responses to CCL2, CCL5, CCL7, and CCL8. Journal of Leukocyte Biology. 74 (2): 243-51.
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    CCL8/MCP-2 related areas, pathways, and other information

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