GFPT1 cDNA ORF Clone, Human, untagged

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GFPT1 cDNA ORF Clone, Human, untagged: General Information

Gene
Species
Human
NCBI Ref Seq
RefSeq ORF Size
2046 bp
Description
Full length Clone DNA of Human glutamine-fructose-6-phosphate transaminase 1.
Plasmid
Promoter
Enhanced CMV promoter
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Ampicillin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.

GFPT1 cDNA ORF Clone, Human, untagged: Alternative Names

CMSTA1 cDNA ORF Clone, Human; GFA cDNA ORF Clone, Human; GFAT cDNA ORF Clone, Human; GFAT1 cDNA ORF Clone, Human; GFAT1m cDNA ORF Clone, Human; GFPT cDNA ORF Clone, Human; GFPT1L cDNA ORF Clone, Human; MSLG cDNA ORF Clone, Human

GFPT1 Background Information

Glutamine:fructose-6-phosphate amidotransferase 1 (GFAT), also known as GFPT1, is a member of the N-terminal nucleophile aminotransferases and the first rate-limiting enzyme for the entry of glucose into the hexosamine biosynthesis pathway (HBP) in mammals. GFAT transfers the amino group from the L-glutamine amide to the D-fructose 6-phosphate, producing glutamic acid and glucosamine 6-phosphate. GFAT exists as a homotetramer in cytoplasm, and is proposed to be most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. The full length of human GFAT contains 1 glutamine amidotransferase type-2 domain which catalyzes amide nitrogen transfer from glutamine to the appropriate substrate, and 2 SIS (Sugar Isomerase) domains found in many phosphosugar isomerases and phosphosugar binding proteins.Two isoforms of gfat have been identified: GFAT1 is predominantly expressed in skeletal muscle, whereas GFAT2 is expressed mainly in the central nervous system.
Full Name
glutamine--fructose-6-phosphate transaminase 1
References
  • McKnight G.L., et al.,(1992), Molecular cloning, cDNA sequence, and bacterial expression of human glutamine:fructose-6-phosphate amidotransferase. J. Biol. Chem. 267:25208-25212.
  • Hillier L.W., et al., (2005), Generation and annotation of the DNA sequences of human chromosomes 2 and 4.Nature 434:724-731.
  • Beausoleil S.A., et al.,(2006), A probability-based approach for high-throughput protein phosphorylation analysis and site localization. Nat. Biotechnol. 24:1285-1292.
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