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Humano HSP90/HSP90AA1 clonación del ADN o clonación génica(vector de clonación), N-Myc Etiqueta

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Human HSP90AA1 Información de producto de clon de cDNA
Gene_bank_ref_id:NM_005348.3
Tamaño de cDNA:2199bp
Descripción de cDNA:Full length Clone DNA of Homo sapiens heat shock protein 90kDa alpha (cytosolic), class A member 1 with N terminal Myc tag.
Sinónimo de gen:HSPN, LAP2, HSP86, HSPC1, HSPCA, Hsp89, Hsp90, HSP89A, HSP90A, HSP90N, HSPCAL1, HSPCAL4, FLJ31884, HSP90AA1
Especie:Human
Vector:pCMV3-N-Myc
Plasmid:
Sitio de restricción:
Secuencia de etiquetas:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Descripción de la secuencia:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Almacenamiento:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Humano HSP90/HSP90AA1 clonación del ADN o clonación génica(vector de clonación), N-Myc Etiqueta on other vectors
Humano HSP90/HSP90AA1 clonación del ADN o clonación génica(vector de clonación), C-GFPSpark EtiquetaHG11445-ACG$245
Humano HSP90/HSP90AA1 clonación del ADN o clonación génica(vector de clonación), C-OFPSpark EtiquetaHG11445-ACR$245
Humano HSP90/HSP90AA1 clonación del ADN o clonación génica(vector de clonación), N-GFPSpark EtiquetaHG11445-ANG$245
Humano HSP90/HSP90AA1 clonación del ADN o clonación génica(vector de clonación), N-OFPSpark EtiquetaHG11445-ANR$245
Humano HSP90/HSP90AA1 clonación del ADN o clonación génica(vector de clonación), C-Flag EtiquetaHG11445-CF$215
Humano HSP90/HSP90AA1 clonación del ADN o clonación génica(vector de clonación), C-His EtiquetaHG11445-CH$215
Humano HSP90/HSP90AA1 clonación del ADN o clonación génica(vector de clonación), C-Myc EtiquetaHG11445-CM$215
Humano HSP90/HSP90AA1 clonación del ADN o clonación génica(vector de clonación), C-HA EtiquetaHG11445-CY$215
Humano HSP90/HSP90AA1 clonación del ADN o clonación génica(Vector de expresión)HG11445-G$75
Humano HSP90/HSP90AA1 clonación del ADN o clonación génica(vector de clonación), N-Flag EtiquetaHG11445-NF$215
Humano HSP90/HSP90AA1 clonación del ADN o clonación génica(vector de clonación), N-His EtiquetaHG11445-NH$215
Humano HSP90/HSP90AA1 clonación del ADN o clonación génica(vector de clonación), N-Myc EtiquetaHG11445-NM$215
Humano HSP90/HSP90AA1 clonación del ADN o clonación génica(vector de clonación), N-HA EtiquetaHG11445-NY$215
Humano HSP90/HSP90AA1 clonación del ADN o clonación génica(vector de clonación)HG11445-UT$215
 Más información sobre los vectores de expresión
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Fondo

Heat shock protein 90 (90 kDa heat-shock protein, HSP90) is a molecular chaperone involved in the trafficking of proteins in the cell. It is a remarkably versatile protein involved in the stress response and in normal homoeostatic control mechanisms. HSP90 interacts with 'client proteins', including protein kinases, transcription factors and others, and either facilitates their stabilization and activation or directs them for proteasomal degradation. By this means, HSP90 displays a multifaceted ability to influence signal transduction, chromatin remodelling and epigenetic regulation, development and morphological evolution. HSP90 operates as a dimer in a conformational cycle driven by ATP binding and hydrolysis at the N-terminus. Disruption of HSP90 leads to client protein degradation and often cell death. Under stressful conditions, HSP90 stabilizes its client proteins and provides protection to the cell against cellular stressors such as in cancer cells. Especially, several oncoproteins act as HSP90 client proteins and tumor cells require higher HSP90 activity than normal cells to maintain their malignancy. For this reason, Hsp90 has emerged as a promising target for anti-cancer drug development.

Referencias
  • Pearl LH, et al. (2008) The Hsp90 molecular chaperone: an open and shut case for treatment. Biochem J. 410(3): 439-53.
  • Hahn JS. (2009) The Hsp90 chaperone machinery: from structure to drug development. BMB Rep. 42(10): 623-30.
  • Holzbeierlein JM, et al. (2010) Hsp90: a drug target? Curr Oncol Rep. 12(2): 95-101.
  • Trepel J, et al. (2010) Targeting the dynamic HSP90 complex in cancer. Nat Rev Cancer. 10(8): 537-49.
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