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Humano LIPG clonación del ADN o clonación génica(vector de clonación), N-Myc Etiqueta

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Human LIPG Información de producto de clon de cDNA
Gene_bank_ref_id:BC060825
Tamaño de cDNA:1503bp
Descripción de cDNA:Full length Clone DNA of Homo sapiens lipase, endothelial with N terminal Myc tag.
Sinónimo de gen:EL, EDL, PRO719, LIPG
Especie:Human
Vector:pCMV3-SP-N-Myc
Plasmid:pCMV3-Myc-LIPG
Sitio de restricción:KpnI + XbaI (6kb + 1.54kb)
Secuencia de etiquetas:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Descripción de la secuencia:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Almacenamiento:The lyophilized plasmid can be stored at room temperature for three months.
Human LIPG Gene Plasmid Map
Human LIPG ORF mammalian expression plasmid, N-Myc tag
Myc Tag Info

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

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Catálogo: HG13734-NM
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  • Human LIPG ORF mammalian expression plasmid, N-Myc tag
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