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Humano LSM3 clonación del ADN o clonación génica(vector de clonación), N-Flag Etiqueta

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    Humano LSM3 Información de producto de clon de cDNA
    Gene_bank_ref_id:BC007055
    Tamaño de cDNA:309bp
    Descripción de cDNA:Full length Clone DNA of Homo sapiens LSM3 homolog, U6 small nuclear RNA associated (S. cerevisiae) with N terminal Flag tag.
    Sinónimo de gen:SMX4, USS2, YLR438C
    Especie:Human
    Vector:pCMV3-N-FLAG
    Plasmid:
    Sitio de restricción:
    Secuencia de etiquetas:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
    Descripción de la secuencia:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with LSM3 qPCR primers for gene expression analysis, HP103204 )
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    Shipping_carrier:Each tube contains lyophilized plasmid.
    Almacenamiento:The lyophilized plasmid can be stored at room temperature for three months.
    FLAG Tag Info

    FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

    A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

    The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

    Humano LSM3 clonación del ADN o clonación génica(vector de clonación), N-Flag Etiqueta on other vectors
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    Fondo

    LSM3 is a member of the snRNP Sm proteins family. Sm-like proteins can be detected in a variety of organisms. They all have the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing. LSM3binds specifically to the 3'-terminal U-tract of U6 snRNA.

    Referencias
  • Gerhard DS. et al., 2004, Genome Res. 14 (10B): 2121-7.
  • Lehner B. et al., 2004, Genome Res. 14 (7): 1315-23.
  • Lehner B. et al., 2004, Genomics. 83 (1): 153-67.
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    Catálogo: HG14554-NF
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