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Humano PLA2G2A clonación del ADN o clonación génica(vector de clonación)

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Human PLA2G2A Información de producto de clon de cDNA
Gene_bank_ref_id:NM_000300.2
Tamaño de cDNA:435bp
Descripción de cDNA:Full length Clone DNA of Homo sapiens phospholipase A2, group IIA (platelets, synovial fluid).
Sinónimo de gen:MOM1, PLA2, PLA2B, PLA2L, PLA2S, PLAS1, sPLA2, PLA2G2A
Especie:Human
Vector:pCMV3-untagged
Plasmid:pCMV3-PLA2G2A
Sitio de restricción:KpnI + XbaI (6.1kb + 0.44kb)
Secuencia de etiquetas:
Descripción de la secuencia:Identical with the Gene Bank Ref. ID sequence except for the point mutation 96 G/C not causing the amino acid variation.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Almacenamiento:The lyophilized plasmid can be stored at room temperature for three months.
Human PLA2G2A Gene Plasmid Map
Human PLA2G2A Gene cDNA Clone (full-length ORF Clone), expression ready, untagged
Humano PLA2G2A clonación del ADN o clonación génica(vector de clonación) on other vectors
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Fondo

Phospholipase A2, membrane associated, also known as Phosphatidylcholine 2-acylhydrolase 2A, Group IIA phospholipase A2, Non-pancreatic secretory phospholipase A2 and PLA2G2A, is a peripheral membrane protein which belongs to the phospholipase A2 family. PLA2G2A is found in many cells and also extracellularly. The membrane-bound and secreted forms of PLA2G2A are identical. PLA2G2A has been proposed to play a role in anti-bacterial defense, inflammation and eicosanoid generation, in clearance of apoptotic cells, and in the Wnt signaling pathway. PLA2G2A is thought to participate in the regulation of the phospholipid metabolism in biomembranes including eicosanoid biosynthesis. PLA2G2A catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. PLA2G2A might be a factor in human colorectal tumorigenesis.

Referencias
  • Praml,C. et al., 1998, Oncogene. 17 (15):2009-12.
  • Fijneman,R.J. et al., 2008,Front Biosci 13 :4144-74.
  • Fijneman,R.J. et al., 2009, Cell Oncol  31 (5):345-56.
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    Catálogo: HG11187-UT
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    • Human PLA2G2A Gene cDNA Clone (full-length ORF Clone), expression ready, untagged
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