|Hoja de datos||Reseñas||Productos relacionados||Protocolos|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human SEZ6L2 ORF mammalian expression plasmid, C-GFPSpark tag||HG13969-ACG|
|Human SEZ6L2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13969-ACR|
|Human SEZ6L2 ORF mammalian expression plasmid, C-Flag tag||HG13969-CF|
|Human SEZ6L2 ORF mammalian expression plasmid, C-His tag||HG13969-CH|
|Human SEZ6L2 ORF mammalian expression plasmid, C-Myc tag||HG13969-CM|
|Human SEZ6L2 ORF mammalian expression plasmid, C-HA tag||HG13969-CY|
|Human SEZ6L2 Gene cDNA clone plasmid||HG13969-G|
|Human SEZ6L2 ORF mammalian expression plasmid, N-Flag tag||HG13969-NF|
|Human SEZ6L2 ORF mammalian expression plasmid, N-His tag||HG13969-NH|
|Human SEZ6L2 ORF mammalian expression plasmid, N-Myc tag||HG13969-NM|
|Human SEZ6L2 ORF mammalian expression plasmid, N-HA tag||HG13969-NY|
|Human SEZ6L2 natural ORF mammalian expression plasmid||HG13969-UT|
|Más información sobre los vectores de expresión|
SEZ6L2, also known as PSK-1, belongs to the SEZ6 family. It contains 3 CUB domains and 5 Sushi (CCP/SCR) domains. SEZ6L2 may contribute to specialized endoplasmic reticulum functions in neurons.SEZ6L2 presents on the surface of lung-cancer cells. SEZ6L2 should be a useful prognostic marker of lung cancers. Increased expression of this gene has been found in lung cancers, and the protein is therefore considered to be a novel prognostic marker for lung cancer.