SMYD3 cDNA ORF Clone, Human, N-DDK (Flag®) tag General Information
Identical with the Gene Bank Ref. ID sequence.
Full length Clone DNA of Human SET and MYND domain containing 3 transcript variant 2 with N terminal Flag tag.
Enhanced CMV promoter
KpnI + XbaI (6kb + 1.15kb)
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
The plasmid is confirmed by full-length sequencing.
Antibiotic in E.coli
Antibiotic in Mammalian cell
Stable or Transient mammalian expression
Storage & Shipping
Each tube contains lyophilized plasmid.
The lyophilized plasmid can be stored at ambient temperature for three months.
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
SMYD3 cDNA ORF Clone, Human, N-DDK (Flag®) tag Validated Images
SMYD3 cDNA ORF Clone, Human, N-DDK (Flag®) tag Alternative Names
bA74P14.1 cDNA ORF Clone, Human;KMT3E cDNA ORF Clone, Human;ZMYND1 cDNA ORF Clone, Human;ZNFN3A1 cDNA ORF Clone, Human
SMYD3 Background Information
SET and MYND domain-containing protein 3, also known as Zinc finger MYND domain-containing protein 1, SMYD3, and ZMYND, is a member of the histone-lysine methyltransferase family. SMYD3 contains one MYND-type zinc finger and one SET domain. SMYD3 is a histone H3 lysine-4-specific methyltransferase. It is expressed in skeletal muscles and testis. It is overexpressed in a majority of colorectal carcinoma (CRC) and hepatocellular carcinoma (HCC). SMYD3 plays an important role in transcriptional regulation in human carcinogenesis. It activates the transcription of a set of downstream genes. Of these downstream genes, there are several oncogenes and genes associated with cell adhesion (including those of N-Myc, CrkL, Wnt10b, L-selectin, CD31 and galectin-4), which have been shown to have effects on cell viability, adhesion, migration and metastasis. Increased SMYD3 expression is essential for the proliferation of breast cancer cells. SMYD3 may be a promising new target of therapeutic intervention for the treatment of cancers or other pathological processes associated with cell adhesion and migration.
SET and MYND domain containing 3
Hamamoto, R. et al., 2006, Cancer Sci. 97 (2): 113-8. Luo, XG. et al., 2007, J Biosci Bioeng. 103 (5): 444-50. Wang, XQ. et al., 2007, Exp Oncol. 29 (1): 71-3. Silva, FP. et al., 2008, Oncogene. 27 (19): 2686-92.