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Ratón MCP-1/CCL2 clonación del ADN o clonación génica(vector de clonación), C-Myc Etiqueta

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Mouse CCL2 Información de producto de clon de cDNA
Gene_bank_ref_id:NM_011333.3
Tamaño de cDNA:447bp
Descripción de cDNA:Full length Clone DNA of Mus musculus chemokine (C-C motif) ligand 2 with C terminal Myc tag.
Sinónimo de gen:JE, HC11, MCAF, MCP1, MCP-1, Scya2, Sigje, SMC-CF, AI323594, Ccl2
Especie:Mouse
Vector:pCMV3-C-Myc
Plasmid:pCMV3-mCCL2-Myc
Sitio de restricción:KpnI + XbaI (6kb + 0.49kb)
Secuencia de etiquetas:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Descripción de la secuencia:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Almacenamiento:The lyophilized plasmid can be stored at room temperature for three months.
Mouse CCL2 Gene Plasmid Map
Mouse CCL2 / MCP-1 natural ORF mammalian expression plasmid, C-Myc tag
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

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  • Mouse CCL2 / MCP-1 natural ORF mammalian expression plasmid, C-Myc tag
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