MAP3K8 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag General Information
Identical with the Gene Bank Ref. ID sequence.
Full length Clone DNA of Mouse mitogen-activated protein kinase kinase kinase 8 with N terminal Flag tag.
Enhanced CMV promoter
KpnI + XbaI(6kb+1.44kb)
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
The plasmid is confirmed by full-length sequencing.
Antibiotic in E.coli
Antibiotic in Mammalian cell
Stable or Transient mammalian expression
Storage & Shipping
Each tube contains lyophilized plasmid.
The lyophilized plasmid can be stored at ambient temperature for three months.
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
MAP3K8 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag Validated Images
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF02). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
MAP3K8 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag Alternative Names
c-COT cDNA ORF Clone, Mouse;Cot cDNA ORF Clone, Mouse;Cot/Tpl2 cDNA ORF Clone, Mouse;Est cDNA ORF Clone, Mouse;Estf cDNA ORF Clone, Mouse;Tpl-2 cDNA ORF Clone, Mouse;Tpl2 cDNA ORF Clone, Mouse
MAP3K8 Background Information
Mitogen-activated protein kinase kinase kinase 8, also known as Cancer Osaka thyroid oncogene, Proto-oncogene c-Cot, Serine/threonine-protein kinase cot, Tumor progression locus 2 and MAP3K8, is a cytoplasm protein which belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and MAP kinase kinase kinase subfamily. MAP3K8 is expressed in several normal tissues and human tumor-derived cell lines. Isoform 1 of MAP3K8 is activated specifically during the S and G2/M phases of the cell cycle. MAP3K8 is required for TLR4 activation of the MEK/ERK pathway. It is able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. MAP3K8 plays a role in the cell cycle. The longer form has some transforming activity, although it is much weaker than the activated cot oncoprotein. MAP3K8 oncogene linked to human endometrial carcinoma suggesting that it may be another molecule involved in human endometrial cancer. MAP3K8 may also be an important mediator of intracellular mechanotransduction in human bone marrow-derived mesenchymal stem cells (MSCs).
mitogen-activated protein kinase kinase kinase 8
Clark,A.M. et al., 2004, Genes Chromosomes Cancer. 41 (2):99-108. Chan,H. et al., 2005, Biochem Biophys Res Commun. 328 (1):198-205. Aparecida Alves,C. et al., 2006, Eur J Gynaecol Oncol. 27 (6):589-93. Mielke,L.A. et al., 2009, J Immunol. 183 (12):7984-93. Glossop,J.R. et al., 2009,Gene Expr Patterns 9 (5):381-8.