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Ratón SLIT2 clonación del ADN o clonación génica(vector de clonación), C-His Etiqueta

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Mouse SLIT2 Información de producto de clon de cDNA
Gene_bank_ref_id:NM_178804.3
Tamaño de cDNA:4566bp
Descripción de cDNA:Full length Clone DNA of Mus musculus slit homolog 2 (Drosophila) with C terminal His tag.
Sinónimo de gen:Slil3, Drad-1, KIAA4141, mKIAA4141, E030015M03Rik, E130320P19Rik, Slit2
Especie:Mouse
Vector:pCMV3-C-His
Plasmid:pCMV3-mSLIT2-His
Sitio de restricción:KpnI + XbaI (6kb + 4.62kb)
Secuencia de etiquetas:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Descripción de la secuencia:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 819 C/T, 1512 T/C, 1716 C/T, 2217 T/C and 3610 A/C not causing the amino acid variation.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Almacenamiento:The lyophilized plasmid can be stored at room temperature for three months.
Mouse SLIT2 Gene Plasmid Map
Mouse SLIT2 Gene cDNA Clone (full-length ORF Clone), expression ready, C-His-tagged
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.

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  • Mouse SLIT2 Gene cDNA Clone (full-length ORF Clone), expression ready, C-His-tagged
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