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Ratón RANKL/OPGL/TNFSF11/CD254 clonación del ADN o clonación génica(vector de clonación), C-Myc Etiqueta

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    Ratón TNFSF11 Información de producto de clon de cDNA
    Gene_bank_ref_id:NM_011613.3
    Tamaño de cDNA:951bp
    Descripción de cDNA:Full length Clone DNA of Mus musculus tumor necrosis factor (ligand) superfamily, member 11 with C terminal Myc tag.
    Sinónimo de gen:ODF, OPG, OPGL, RANKL, Ly109l, Trance
    Especie:Mouse
    Vector:pCMV3-C-Myc
    Plasmid:
    Sitio de restricción:
    Secuencia de etiquetas:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
    Descripción de la secuencia:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with TNFSF11 qPCR primers for gene expression analysis, MP200365 )
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    Shipping_carrier:Each tube contains lyophilized plasmid.
    Almacenamiento:The lyophilized plasmid can be stored at room temperature for three months.
    Myc Tag Info

    A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

    A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

    The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

    Ratón RANKL/OPGL/TNFSF11/CD254 clonación del ADN o clonación génica(vector de clonación), C-Myc Etiqueta on other vectors
    Ratón RANKL/OPGL/TNFSF11/CD254 clonación del ADN o clonación génica(vector de clonación), C-GFPSpark EtiquetaMG50343-ACG$225
    Ratón RANKL/OPGL/TNFSF11/CD254 clonación del ADN o clonación génica(vector de clonación), C-OFPSpark EtiquetaMG50343-ACR$225
    Ratón RANKL/OPGL/TNFSF11/CD254 clonación del ADN o clonación génica(vector de clonación), C-Flag EtiquetaMG50343-CF$195
    Ratón RANKL/OPGL/TNFSF11/CD254 clonación del ADN o clonación génica(vector de clonación), C-His EtiquetaMG50343-CH$195
    Ratón RANKL/OPGL/TNFSF11/CD254 clonación del ADN o clonación génica(vector de clonación), C-Myc EtiquetaMG50343-CM$195
    Ratón RANKL/OPGL/TNFSF11/CD254 clonación del ADN o clonación génica(vector de clonación), C-HA EtiquetaMG50343-CY$195
    Ratón RANKL/OPGL/TNFSF11/CD254 clonación del ADN o clonación génica(Vector de expresión)MG50343-M$75
    Ratón RANKL/OPGL/TNFSF11/CD254 clonación del ADN o clonación génica(vector de clonación), N-Flag EtiquetaMG50343-NF$195
    Ratón RANKL/OPGL/TNFSF11/CD254 clonación del ADN o clonación génica(vector de clonación), N-His EtiquetaMG50343-NH$195
    Ratón RANKL/OPGL/TNFSF11/CD254 clonación del ADN o clonación génica(vector de clonación), N-Myc EtiquetaMG50343-NM$195
    Ratón RANKL/OPGL/TNFSF11/CD254 clonación del ADN o clonación génica(vector de clonación), N-HA EtiquetaMG50343-NY$195
    Ratón RANKL/OPGL/TNFSF11/CD254 clonación del ADN o clonación génica(vector de clonación)MG50343-UT$195
     Más información sobre los vectores de expresión
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    Fondo

    Tumor necrosis factor ligand superfamily member 11, also known as Receptor activator of nuclear factor kappa-B ligand, Osteoprotegerin ligand, TNFSF11, RANKL, TRANCE, OPGL and CD254, is a single-pass type II membrane protein which belongs to the tumor necrosis factor family. The receptor activator of nuclear factor-kappaB ligand (RANKL), its cognate receptor RANK, and its natural decoy receptor osteoprotegerin have been identified as the final effector molecules of osteoclastic bone resorption. RANK and RANKL are key regulators of bone remodeling and regulate T cell/dendritic cell communications, and lymph node formation. Moreover, RANKL and RANK are expressed in mammary gland epithelial cells and control the development of a lactating mammary gland during pregnancy. Genetically, RANKL and RANK are essential for the development and activation of osteoclasts and bone loss in response to virtually all triggers tested. Inhibition of RANKL function via the natural decoy receptor osteoprotegerin (OPG, TNFRSF11B) prevents bone loss in postmenopausal osteoporosis and cancer metastases. Importantly, RANKL appears to be the pathogenetic principle that causes bone and cartilage destruction in arthritis. RANK-RANKL signaling not only activates a variety of downstream signaling pathways required for osteoclast development, but crosstalk with other signaling pathways also fine-tunes bone homeostasis both in normal physiology and disease. In addition, RANKL and RANK have essential roles in lymph node formation, establishment of the thymic microenvironment, and development of a lactating mammary gland during pregnancy.

    Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

    Referencias
  • Takayanagi H, et al. (2002) Signaling crosstalk between RANKL and interferons in osteoclast differentiation. Arthritis Res. 4 Suppl 3: S227-32.
  • Nakashima T, et al. (2003) RANKL and RANK as novel therapeutic targets for arthritis. Curr Opin Rheumatol. 15(3): 280-7.
  • Schwarz EM, et al. (2007) Clinical development of anti-RANKL therapy. Arthritis Res Ther. 9 Suppl 1: S7.
  • Leibbrandt A, et al. (2008) RANK/RANKL: regulators of immune responses and bone physiology. Ann N Y Acad Sci. 1143: 123-50.
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