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Humano PCDHGC4 clonación del ADN o clonación génica(Vector de expresión)

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Human PCDHGC4 Información de producto de clon de cDNA
Gene_bank_ref_id:BC101035
Tamaño de cDNA:2616bp
Descripción de cDNA:Full length Clone DNA of Homo sapiens protocadherin gamma subfamily C, 4.
Sinónimo de gen:PCDH-GAMMA-C4
Especie:Human
Vector:pGEM-T Vector
Plasmid:pGEM-PCDHGC4
Sitio de restricción:
Secuencia de etiquetas:
Descripción de la secuencia:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Almacenamiento:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
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