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Rat Clusterin/Apolipoprotein J/Apo-J clonación del ADN o clonación génica(vector de clonación), N-Flag Etiqueta

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    Rata CLU Información de producto de clon de cDNA
    Gene_bank_ref_id:BC061534.1
    Tamaño de cDNA:1344bp
    Descripción de cDNA:Full length Clone DNA of Rattus norvegicus clusterin with N terminal Flag tag.
    Sinónimo de gen:APOJ,CLI,DAG,RATTRPM2B,SGP-2,SGP2,SP-40,SP40,TRPM-2,Trpm2,TRPM2B,Trpmb
    Especie:Rat
    Vector:pCMV3-SP-N-FLAG
    Plasmid:
    Sitio de restricción:
    Secuencia de etiquetas:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
    Descripción de la secuencia:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    Shipping_carrier:Each tube contains lyophilized plasmid.
    Almacenamiento:The lyophilized plasmid can be stored at room temperature for three months.
    FLAG Tag Info

    FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

    A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

    The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

    Rat Clusterin/Apolipoprotein J/Apo-J clonación del ADN o clonación génica(vector de clonación), N-Flag Etiqueta on other vectors
    Rat Clusterin/Apolipoprotein J/Apo-J clonación del ADN o clonación génica(vector de clonación), C-GFPSpark EtiquetaRG81601-ACG$225
    Rat Clusterin/Apolipoprotein J/Apo-J clonación del ADN o clonación génica(vector de clonación), C-OFPSpark EtiquetaRG81601-ACR$225
    Rat Clusterin/Apolipoprotein J/Apo-J clonación del ADN o clonación génica(vector de clonación), C-Flag EtiquetaRG81601-CF$195
    Rat Clusterin/Apolipoprotein J/Apo-J clonación del ADN o clonación génica(vector de clonación), C-His EtiquetaRG81601-CH$195
    Rat Clusterin/Apolipoprotein J/Apo-J clonación del ADN o clonación génica(vector de clonación), C-Myc EtiquetaRG81601-CM$195
    Rat Clusterin/Apolipoprotein J/Apo-J clonación del ADN o clonación génica(vector de clonación), C-HA EtiquetaRG81601-CY$195
    Rat Clusterin/Apolipoprotein J/Apo-J clonación del ADN o clonación génica(Vector de expresión)RG81601-G$75
    Rat Clusterin/Apolipoprotein J/Apo-J clonación del ADN o clonación génica(vector de clonación), N-Flag EtiquetaRG81601-NF$195
    Rat Clusterin/Apolipoprotein J/Apo-J clonación del ADN o clonación génica(vector de clonación), N-His EtiquetaRG81601-NH$195
    Rat Clusterin/Apolipoprotein J/Apo-J clonación del ADN o clonación génica(vector de clonación), N-Myc EtiquetaRG81601-NM$195
    Rat Clusterin/Apolipoprotein J/Apo-J clonación del ADN o clonación génica(vector de clonación), N-HA EtiquetaRG81601-NY$195
    Rat Clusterin/Apolipoprotein J/Apo-J clonación del ADN o clonación génica(vector de clonación)RG81601-UT$195
     Más información sobre los vectores de expresión
    Product nameProduct name
    Fondo

    Clusterin, also known as complement-associated protein SP-40, Complement cytolysis inhibitor, Apolipoprotein J, Testosterone-repressed prostate message 2, Aging-associated gene 4 protein, CLU and APOJ, is a secreted protein which belongs to the clusterin family. Clusterin/Apolipoprotein J/Apo-J is an enigmatic glycoprotein with a nearly ubiquitous tissue distribution and an apparent involvement in biological processes ranging from mammary gland involution to neurodegeneration in Alzheimer's disease. Its major form, a heterodimer, is secreted and present in physiological fluids, but truncated forms targeted to the nucleus have also been identified. Clusterin/Apolipoprotein J/Apo-J is a widely distributed glycoprotein with a wide range of biologic properties. A prominent and defining feature of clusterin is its marked induction in such disease states as glomerulonephritis, cystic renal disease, renal tubular injury, neurodegenerative conditions, atherosclerosis, and myocardial infarction. Upregulation of clusterin mRNA and protein levels detected in diverse disease states and in in vitro systems have led to suggestions that it functions in membrane lipid recycling, in apoptotic cell death, and as a stress-induced secreted chaperone protein, amongst others.

    Referencias
  • Silkensen JR, et al. (1994) The role of clusterin in tissue injury. Biochem Cell Biol. 72(11-12): 483-8.
  • Naik RR, et al. (2002) Biomimetic synthesis and patterning of silver nanoparticles. Nat Mater. 1(3): 169-72.
  • Djeu JY, et al. (2009) Clusterin and chemoresistance. Adv Cancer Res. 105: 77-92.
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    Catálogo: RG81601-NF
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