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Rat IL4/IL-4/Interleukin-4 clonación del ADN o clonación génica(vector de clonación)

  • Rat IL4 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged
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Rata IL4 Información de producto de clon de cDNA
Gene_bank_ref_id:NM_201270.1
Tamaño de cDNA:444bp
Descripción de cDNA:Full length Clone DNA of Rattus norvegicus interleukin 4.
Sinónimo de gen:Il4e12, Il4
Especie:Rat
Vector:pCMV3-untagged
Plasmid:pCMV3-ratIL4
Sitio de restricción:KpnI + XbaI (6.1kb + 0.44kb)
Secuencia de etiquetas:
Descripción de la secuencia:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with IL4 qPCR primers for gene expression analysis, RP300222 )
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Ampicillin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Almacenamiento:The lyophilized plasmid can be stored at room temperature for three months.
Rata IL4 Gene Plasmid Map
Rat IL4 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged
Rat IL4/IL-4/Interleukin-4 clonación del ADN o clonación génica(vector de clonación) on other vectors
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Interleukin-4, also known as IL4, is a secreted protein which belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4 / IL4 also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and for the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes as well as of other cell types. However, studies show that double mutant (Q116D, Y119D) of the murine IL4 protein (QY), both glutamine 116 and tyrosine 119, which binds to the IL4 receptor alpha, completely inhibites in a dose-dependent manner the IL4-induced proliferation of lipopolysaccharide-stimulated murine splenic B-cells, of the murine T cell line CTLL-2, and of the murine pre-B-cell line BA/F3. QY also inhibited the IL4-stimulated up-regulation of CD23 expression by lipopolysaccharide-stimulated murine splenic B-cells and abolished tyrosine phosphorylation of the transcription factor Stat6 and the tyrosine kinase Jak3 in IL4-stimulated BA/F3 cells.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Referencias
  • Grunewald SM. et al., 1998, J Immunol. 160 (8): 4004-9.
  • Susanne M. et al, 1997, THE JOURNAL OF BIOLOGICAL CHEMISTRY. 272 (3): 1480-3.
  • Nishikubo K. et al., 2003, Gene Ther. 10 (26): 2119-25.
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