|Hoja de datos||Reseñas||Productos relacionados||Protocolos|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Rat KIT ORF mammalian expression plasmid, C-GFPSpark tag||RG80321-ACG|
|Rat KIT ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80321-ACR|
|Rat KIT ORF mammalian expression plasmid, C-Flag tag||RG80321-CF|
|Rat KIT ORF mammalian expression plasmid, C-His tag||RG80321-CH|
|Rat KIT ORF mammalian expression plasmid, C-Myc tag||RG80321-CM|
|Rat KIT ORF mammalian expression plasmid, C-HA tag||RG80321-CY|
|Rat KIT Gene cDNA clone plasmid||RG80321-G|
|Rat KIT ORF mammalian expression plasmid, N-Flag tag||RG80321-NF|
|Rat KIT ORF mammalian expression plasmid, N-His tag||RG80321-NH|
|Rat KIT ORF mammalian expression plasmid, N-Myc tag||RG80321-NM|
|Rat KIT ORF mammalian expression plasmid, N-HA tag||RG80321-NY|
|Rat KIT natural ORF mammalian expression plasmid||RG80321-UT|
|Más información sobre los vectores de expresión|
C-Kit is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). c-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains.and 1 protein kinase domain. It belongs to the protein kinase superfamily, tyr protein kinase family and CSF-1/PDGF receptor subfamily. C-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. C-Kit has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase. Antibodies to c-Kit are widely used in immunohistochemistry to help distinguish particular types of tumour in histological tissue sections. It is used primarily in the diagnosis of GISTs. In GISTs, c-Kit staining is typically cytoplasmic, with stronger accentuation along the cell membranes. C-Kit antibodies can also be used in the diagnosis of mast cell tumours and in distinguishing seminomas from embryonal carcinomas. Mutations in c-Kit gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Defects in KIT are a cause of acute myelogenous leukemia (AML). AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development. Note=Somatic mutations that lead to constitutive activation of KIT are detected in AML patients.