Anti-Cofilin Antibody

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Anti-Cofilin Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-Cofilin Antibody
Validated applications
WB,ELISA,IHC-P
Species reactivity
Reacts with: Human
Specificity
Human Cofilin
Immunogen
Recombinant Human CFL1 / cofilin Protein (Catalog#14544-H07E)
Preparation
Produced in rabbits immunized with purified, recombinant Human CFL1 / cofilin (rh CFL1 / cofilin; Catalog#14544-H07E; P23528; Met1-Leu166). CFL1 / cofilin specific IgG was purified by Human CFL1 / cofilin affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Cofilin Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
ELISA 1:5000-1:10000
IHC-P 1:10000-1:20000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Cofilin Antibody (Rabbit Polyclonal antibody) Images

Immunochemical staining of human CFL1 in human hepatoma with rabbit polyclonal antibody (1:20000, formalin-fixed paraffin embedded sections).
Immunochemical staining of human CFL1 in human breast carcinoma with rabbit polyclonal antibody (1:20000, formalin-fixed paraffin embedded sections).

Anti-CFL1 rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lane B: A431 Whole Cell Lysate

Lane C: MCF7 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:19 kDa

Observed band size:19 kDa

Anti-Cofilin Antibody: Alternative Names

Anti-CFL Antibody; Anti-HEL-S-15 Antibody

Cofilin Background Information

CFL1, also known as n-cofilin, is a member of the ADF/Cofilin family. This family comprises three genes: CFL1, CFL2 and DSTN (destrin). ADF/Cofilin family members bind G-actin monomers and depolymerize actin filaments through two mechanisms: severing and increasing the off-rate for actin monomers from the pointed end. Cofilin also binds with other proteins such as myosin, tropomyosin, α-actinin, gelsolin and scruin. These proteins compete with cofilin for actin binding. Сofilin also plays a role in innate immune response. CFL1 contains 1 ADF-H domain and is widely distributed in various tissues. It is important for normal progress through mitosis and normal cytokinesis.
Full Name
cofilin 1 (non-muscle)
References
  • Lappalainen P. et al., 1997, Nature. 388 (6637): 78-82.
  • Ichetovkin I. et al., 2000, Cell Motil. 45 (4): 293-306.
  • Carlier MF. et al., 1997, J Cell Biol. 136 (6): 1307-22.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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