Recombinant Proteins

The Sino Biological R&D team has over 10 years of experience in recombinant protein research and development. Over the years our team has generated more than 10,000 recombinant proteins with more than 6,000 recombinant proteins currently in stock covering most hot research areas such as immune checkpoints, antibody drug targets, CAR-T cell therapy targets, Fc receptors, influenza viral proteins and cytokines.
Exhibiting properties of high purity and validated bioactivity, Sino Biological recombinant proteins can support your scientific research from biopharmaceutical target discovery, protein structure and function analysis, cell therapy and recombinant enzyme characterization.

Featured Recombinant Proteins

  • SARS-CoV-2 (2019-nCoV) Antigens

    Sino Biological Inc. has developed a panel of recombinant antigens for SARS-CoV-2, including the N (nucleocapsid) protein, S protein, the S1 and S2 subunits of the S protein, and the RBD domain of the S proteins, Plpro protein.

  • Immune Checkpoint proteins

    Covers most hot immune checkpoint proteins including PD-1/ PD-L1 and CTLA-4 approved antibody drugs as well as prospective drug candidates such as TIM-3, CD47, GITR, etc.
     

  • CAR-T cell therapy targets

    Covers many hot cell therapy targets including CA9, EGFR and CD274 approved antibody drugs as well as prospective drug candidates such as CD19, CD33, IL3RA, EPCAM, TNFRSF17, etc.
     

  • Monoclonal Antibody Therapeutic Targets

    Covers many antibody therapeutic targets including PD-1 and CD20 approved antibody drugs as well as prospective candidates such as PD-1, Her2 / ERBB2, EGFR, c-MET, IL6, IL17, TNF-alpha……

  • Cytokine & Growth Factor

    GMP-grade proteins available.
    Covers the entire cytokine family, including the interleukins, chemokines and TNFs. A special focus is on the biopharmaceutical industry and cell therapy strategies.

  • Fc Receptors

    Covers the vast majority of Fc receptors across multiple species. Each receptor is verified for high bioactivity of binding to immunoglobulins.
     

  • Biotinylated Protein

    Covers such classes as mAb drug targets, CAR-T related targets and vital Fc receptors. Each protein is confirmed for retention of functionality after biotinylation.
     

A Global Leader in Recombinant Protein R & D

Advanced Technology Platforms

  • • Five expression systems to support production
  • • Proteins generated from multiple species to support animal models and drug development
  • • 70 automatic bioreactors
  • • 2L-2000L bioreactors to ensure high throughout manufacture
  • • GMP-level production laboratory and facilities
  • • Milligram-to-gram level protein production

Strict Quality Control Systems

  • • Over 1000 methods for bio-activity confirmation and analysis
  • • State-of-the-art analytical equipment ensures validated bioactivity
  • • N-terminal amino acid sequence analysis capabilities
  • • High stability to ensure reproducible experimentation
  • • Qualified raw materials to ensure animal-free production

Premium Recombinant Protein Products

  • • >85% expressed by eukaryotic cells, close to natural structure
  • • Validated bioactivity
  • • Confirmation of low endotoxin levels: <0.1 EU per μg by LAL
  • • Confirmation of high purity: >95% by SDS-PAGE or HPLC
  • • Carrier-Free: Dissolved in PBS, without BSA

Highly Pure Recombinant Proteins

  • The purity of human TNFα protein (Cat. No. GMP-10602-HNAE) was >97% determined by SEC-HPLC and >96% determined by SDS-PAGE
  • Fig .1 The purity of human TNFα protein (Cat. No. GMP-10602-HNAE) was >97% determined by SEC-HPLC and >96% determined by SDS-PAGE

Recombinant Protein with High Bioactivities

  • The comparison of VEGF165 bioactivity from Sino Biological (EC50=1.4 ng/ml) and another brand (EC50=4 ng/ml) in cell proliferation assays using HUVEC
  • Fig .2 The comparison of VEGF165 bioactivity from Sino Biological (EC50=1.4 ng/ml) and another brand (EC50=4 ng/ml) in cell proliferation assays using HUVEC.

Recombinant Protein with High Binding Activities

  • The PD-1 (Cat. No. 10377-H03H) binding ability to PD-L2 was measured in a functional ELISA. ED50=0.94 μg/ml
  • Fig .3 The PD-1 (Cat. No. 10377-H03H) binding ability to PD-L2 was measured in a functional ELISA. ED50=0.94 μg/ml.

Custom Recombinant Protein Production

Recombinant Protein Production Service Description
We offer one-stop services for recombinant protein production using our advanced protein expression platform technologies. Our full services include protein gene synthesis, protein codon optimization, protein expression vector design, large scale cell culture and fermentation, protein purification and protein quality control testing. We offer significant cost saving advantages and record speed in protein expression and bulk production. We also have extensive experience and expertise in the design and execution of various types of recombinant protein purification projects, with or without purification tags.

Sino Biological has in-house capacity to support 1000-2000 recombinant protein development and bulk production projects per year. We have the ability to perform rapid protein expression and purification process development and conduct process scaleup studies as needed. We can meet protein production requirements for both high-throughput and large-scale bulk production requirements. Over the years, we have successfully produced over 10,000 recombinant proteins in bulk (in the range of milligram to gram purified proteins) with over 6,000 of these now in-stock. This unprecedented capacity can be utilized by our customers worldwide to accelerate their basic research, structural biology studies, product discovery initiatives and product development timelines.

We are committed to become the world's most cost-effective centralized lab for recombinant protein and monoclonal antibody bulk production to serve the life science community and biotech industry worldwide. We strive to change the way the life science research community and industry are generating and sourcing their proteins.

More about Sino Biological's Recombinant Protein Production Service

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