CD160 Protein

CD160 Protein Overview

CD160 reagents

Anumanthan et al. (1998) isolated the gene encoding BY55 by COS cell expression cloning using mRNA pooled from natural killer (NK) cells of 4 donors. The sequence of the 1.3-kb cDNA transcript predicts a cysteine-rich, glycosylphosphatidylinositol (GPI)-anchored protein of 181 amino acids. The protein contains 2 N-linked glycosylation sites and a single extracellular Ig-like (see 147100, 300137) domain weakly homologous to MHC binding receptors collectively referred to as killer inhibitory receptors (KIR). Northern blot analysis detected BY55 in only spleen, peripheral blood, and small intestine. BY55 mRNA expression is restricted to functional NK and T cytotoxic lymphocytes, specifically CD56(dim)/CD16+ NK cells and CD8+,CD28-,CD11b+ peripheral blood or intestinal intraepithelial T lymphocytes. No expression was found in B-lymphocyte cell lines, T-cell leukemia cell lines, or a myeloid cell line. Maiza et al. (1993) showed that a monoclonal antibody to BY55 immunoprecipitated an 80-kD protein as well as a less abundant 27-kD protein from an NK-like cell line. Anumanthan et al. (1998) found that by reducing the immunoprecipitated BY55 protein with dithiothreitol and iodoacetamide, the relative amount of 27- to 80-kD protein could be increased, suggesting that the smaller predicted protein size is correct and that BY55 is a tightly disulfide-linked multimer. Phosphatidylinositol-specific phospholipase C cleaved BY55 from NK-like cell lines, confirming that BY55 protein is anchored to the cell surface via a GPI linkage.

CD160 protein name

Recommended name
CD160 antigen
Aliases
BY55, NK1, NK28
Alternative name
Natural killer cell receptor BY55 CD antigen CD160

CD160 Gene family protein

CD160 Protein Sequence

Species Human CD160 protein
Length 181
Mass (Da) 19810
Sequence Human CD160 protein sequence
Species Mouse CD160 protein
Length 185
Mass (Da) 20570
Sequence Mouse CD160 protein sequence
Species
Length
Mass (Da)
Sequence

CD160 Protein Molecular Weight & PI

CD160 antigen precursor (Natural killer cell receptor BY55) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 27-159 CD160 antigen.

Molecular weight (Da)

14795.71

Theoretical pI

7.84

CD160 Protein Interaction

Recombinant CD160 Protein Feature

CD160 Protein, Human, Recombinant (His Tag)

High Purity
> 90 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
High Activity
Measured by its ability to bind with biotinylated human HVEM-Fch (Cat:10334-H03H) in a functional ELISA.

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