CD4 Antibody

CD4 Antibody Overview

There are 40 CD4 antibodies which are validated in multiple tissues with various applications, including ELISA, WB, FCM, IHC-P, ICC/IF, IP, ELISA(Det), ELISA(Cap). There are 15 CD4 antibody for ELISA, 4 CD4 antibody for WB, 20 CD4 antibody for FCM, 5 CD4 antibody for IHC-P, 1 CD4 antibody for ICC/IF, 1 CD4 antibody for IP, 1 CD4 antibody for ELISA(Det), 1 CD4 antibody for ELISA(Cap). Among all these CD4 antibodies, there are 20 anti-CD4 mouse monoclonal antibodies , 13 anti-CD4 rabbit monoclonal antibodies , 7 anti-CD4 rabbit polyclonal antibodies . All the CD4 anbodies are produced in house and all are in stock. CD4 antibody customerized service is available.

CD4 gene family antibody

CD4 Antibody ELISA

CD4 antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD4 (rh CD4; Catalog#10400-H08H; NP_000607.1; Met 1-Trp 390). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD4 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

CD4 Antibody western blot

CD4 Antibody western blot Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD4 (rh CD4; Catalog#10400-H08H; NP_000607.1; Met1-Trp390). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD4 Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

CD4 Antibody flow cytometry

CD4 Antibody flow cytometry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Ferret CD4 (rF CD4; Catalog#60003-F08H; ABS50090.1; Met 1-Leu 401). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD4 Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

CD4 Antibody immunohistochemistry

CD4 Antibody immunohistochemistry Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human CD4 (rh CD4; Catalog#10400-H08H; NP_000607.1; Met 1-Trp 390).

CD4 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:100-1:500
Notes  

CD4 Antibody immunoprecipitation

CD4 Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Mouse CD4 (rM CD4; Catalog#50134-M08H; NP_038516.1; Met1-Thr394). CD4 specific IgG was purified by Mouse CD4 affinity chromatography.

CD4 Antibody immunoprecipitation Validated

Application IP
Dilution 0.1-0.5 μL/mg of lysate
Notes  

CD4 antibody citations

Title
An autoreactive antibody from an SLE/HIV-1 individual broadly neutralizes HIV-1
Year
2014
Author
M Bonsignori, K Wiehe, SK Grimm…
Journal
The Journal of Clinical Investigation
Title
An autoreactive antibody from an SLE/HIV-1 individual broadly neutralizes HIV-1
Year
2014
Author
M Bonsignori, K Wiehe, SK Grimm…
Journal
The Journal of Clinical Investigation
Title
Stockpiled pre-pandemic H5N1 influenza virus vaccines with AS03 adjuvant provide cross-protection from H5N2 clade 2.3.4.4 virus challenge in ferrets
Year
2017
Author
Sun, X;Belser, JA;Pulit-Penaloza, JA;Creager, HM;Guo, Z;Jefferson, SN;Liu, F;York, IA;Stevens, J;Maines, TR;Jernigan, DB;Katz, JM;Levine, MZ;Tumpey, TM;
Journal
Virology
Title
The immune correlates of protection for an avian influenza H5N1 vaccine in the ferret model using oil-in-water adjuvants
Year
2017
Author
Wong, SS;Duan, S;DeBeauchamp, J;Zanin, M;Kercher, L;Sonnberg, S;Fabrizio, T;Jeevan, T;Crumpton, JC;Oshansky, C;Sun, Y;Tang, L;Thomas, P;Webby, R;
Journal
Sci Rep
Title
Extensive T cell cross-reactivity between diverse seasonal influenza strains in the ferret model
Year
2018
Author
Reber, AJ;Music, N;Kim, JH;Gansebom, S;Chen, J;York, I;
Journal
Sci Rep
Title
TGFβ drives immune evasion in genetically reconstituted colon cancer metastasis
Year
2018
Author
Tauriello, DVF;Palomo-Ponce, S;Stork, D;Berenguer-Llergo, A;Badia-Ramentol, J;Iglesias, M;Sevillano, M;Ibiza, S;Cañellas, A;Hernando-Momblona, X;Byrom, D;Matarin, JA;Calon, A;Rivas, EI;Nebreda, AR;Riera, A;Attolini, CS;Batlle, E;
Journal
Nature
Title
The Cdx2 homeobox gene suppresses intestinal tumorigenesis through non-cell-autonomous mechanisms
Year
2018
Author
Balbinot, C;Armant, O;Elarouci, N;Marisa, L;Martin, E;De Clara, E;Onea, A;Deschamps, J;Beck, F;Freund, JN;Duluc, I;
Journal
J. Exp. Med.