VEGFR2 / KDR Protein, Mouse, Recombinant (His Tag), Biotinylated

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VEGFR2 / KDR Protein, Mouse, Recombinant (His Tag), Biotinylated: Product Information

Purity
> 90 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Activity
1.Measured by its binding ability in a functional ELISA.
2.Immobilized human VEGF165(Cat:11066-HNAB) at 10μg/mL (100μL/well) can bind biotinylated mouse KDR(Cat:50998-M08H-B), the EC50 of biotinylated mouse KDR is 40-130ng/mL.
Protein Construction
A DNA sequence encoding the mouse KDR (P35918-1) extracellular domain (Met1-Glu762) was expressed with a C-terminal polyhistidine tag. The purified protein was biotinylated in vitro.
Accession#
Expressed Host
HEK293 Cells
Species
Mouse
Predicted N Terminal
Ala 20
Molecule Mass
The recombinant mouse KDR consists of 754 amino acids and predicts a molecular mass of 84.5 KDa.
Formulation
Lyophilized from sterile PBS.
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

VEGFR2 / KDR Protein, Mouse, Recombinant (His Tag), Biotinylated: Images

VEGFR2 / KDR Protein, Mouse, Recombinant (His Tag), Biotinylated: Alternative Names

6130401C07 Protein, Mouse; Flk-1 Protein, Mouse; Flk1 Protein, Mouse; Krd-1 Protein, Mouse; Ly73 Protein, Mouse; sVEGFR-2 Protein, Mouse; VEGFR-2 Protein, Mouse; VEGFR2 Protein, Mouse

VEGFR2 / KDR Background Information

VEGFR2, also called as KDR or Flk-1, is identified as the receptor for VEGF and VEGFC and an early marker for endothelial cell progenitors, whose expression is restricted to endothelial cells in vivo. VEGFR2 was shown to be the primary signal transducer for angiogenesis and the development of pathological conditions such as cancer and diabetic retinopathy. It has been shown that VEGFR2 is expressed mainly in the endothelial cells, and the expression is upregulated in the tumor vasculature. Thus the inhibition of VEGFR2 activity and its downstream signaling are important targets for the treatment of diseases involving angiogenesis. VEGFR2 transduces the major signals for angiogenesis via its strong tyrosine kinase activity. However, unlike other representative tyrosine kinase receptors, VEGFR2 does not use the Ras pathway as a major downstream signaling but rather uses the phospholipase C-protein kinase C pathway to signal mitogen-activated protein (MAP)-kinase activation and DNA synthesis. VEGFR2 is a direct and major signal transducer for pathological angiogenesis, including cancer and diabetic retinopathy, in cooperation with many other signaling partners; thus, VEGFR2 and its downstream signaling appear to be critical targets for the suppression of these diseases. VEGF and VEGFR2-mediated survival signaling is critical to endothelial cell survival, maintenance of the vasculature and alveolar structure and regeneration of lung tissue. Reduced VEGF and VEGFR2 expression in emphysematous lungs has been linked to increased endothelial cell death and vascular regression.
Full Name
kinase insert domain receptor
Research Areas
References
  • Shibuya M. (2006) Vascular endothelial growth factor (VEGF)-Receptor2: its biological functions, major signaling pathway, and specific ligand VEGF-E. Endothelium. 13(2): 63-9.
  • Marwick JA, et al. (2010) Cigarette smoke regulates VEGFR2-mediated survival signaling in rat lungs. J Inflamm (Lond). 7(1): 11.
  • Bruns AF, et al. (2010) Ligand-stimulated VEGFR2 signaling is regulated by co-ordinated trafficking and proteolysis. Traffic. 11(1): 161-74.
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