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4-1BB/TNFSF9 Protein, Human, Recombinant (ECD, hFc Tag)

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4-1BB/TNFSF9 Protein, Human, Recombinant (ECD, hFc Tag): Product Information

Purity
> 85 % as determined by SDS-PAGE.
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Activity
1.Measured by its binding ability in a functional ELISA.
2.Immobilized human TNFRSF9 (Cat:10041-H08H) at 10μg/mL (100μL/well) can bind human TNFSF9 (Cat:15693-H01H),the EC50 of human TNFSF9 is 7-40ng/mL.
Protein Construction
A DNA sequence encoding the human TNFSF9 (NP_003802.1) (Arg71-Glu254) was expressed with the Fc region of human IgG1 at the N-terminus.
Accession#
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Glu
Molecule Mass
The recombinant human TNFSF9 consists 444 amino acids and predicts a molecular mass of 47.9 kDa.
Formulation
Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

4-1BB/TNFSF9 Protein, Human, Recombinant (ECD, hFc Tag): Images

1.Measured by its binding ability in a functional ELISA.  2.Immobilized human TNFRSF9 (Cat:10041-H08H) at 10μg/mL (100μL/well) can bind human TNFSF9 (Cat:15693-H01H),the EC50 of human TNFSF9 is 7-40ng/mL.

4-1BB/TNFSF9 Protein, Human, Recombinant (ECD, hFc Tag): Alternative Names

4-1BB-L Protein, Human; 4-1BBL Protein, Human; CD137L Protein, Human

4-1BB/TNFSF9 Background Information

4-1BBL is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. It is shown to be a type II surface glycoprotein belonging to the TNF superfamily. Expression of 4-1BBL is restricted to APCs, such as dendritic cells, macrophages, and activated B cells. Members of the TNF-TNF receptor superfamily have been shown to play critical roles in regulating cellular activation, differentiation and apoptosis. Several studies have reported that 4-1BBL/4-1BB interaction provided a co-stimulatory signal to T cells, and increased T cell proliferation and cytokines production. In additon, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.
Full Name
tumor necrosis factor (ligand) superfamily, member 9
References
  • Cheung CT, (2007) Neutralizing anti-4-1BBL treatment improves cardiac function in viral myocarditis. Lab Invest. 87(7): 651-61.
  • Wang C, et al. (2009) Immune regulation by 4-1BB and 4-1BBL: complexities and challenges. Immunol Rev. 229(1): 192-215.
  • Habib-Agahi M, et al. (2009) 4-1BBL costimulation retrieves CD28 expression in activated T cells. Cell Immunol. 256(1-2): 39-46.
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