Anti-SIRP alpha Antibody

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Anti-SIRP alpha Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-SIRP alpha Antibody
Validated applications
WB,ELISA,IHC-P
Species reactivity
Reacts with: Rat
Specificity
Rat SIRP alpha
Immunogen
Recombinant Rat SIRP alpha / CD172a protein (Catalog#80270-R08H)
Preparation
Produced in rabbits immunized with purified, recombinant Rat SIRP alpha / CD172a (Catalog#80270-R08H; P97710; Met1-Asn373). SIRP alpha / CD172a specific IgG was purified by Rat SIRP alpha / CD172a affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-SIRP alpha Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
ELISA 1:5000-1:10000
IHC-P 1:2500-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-SIRP alpha Antibody (Rabbit Polyclonal antibody) Images

Immunochemical staining of rat SIRPA in rat kidney with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).
Immunochemical staining of rat SIRPA in rat lung with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).

Anti-SIRPA rabbit polyclonal antibody at 1:500 dilution

Lane A: NIH-3T3 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:55 kDa

Observed band size:55 kDa

(We are unsure as to the identity of these extra bands.)

SIRP alpha Background Information

Tyrosine-protein phosphatase non-receptor type substrate 1, also known as SHP substrate 1, Inhibitory receptor SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, Macrophage fusion receptor, CD172 antigen-like family member A, SIRPA and CD172a, is a single-pass type I membrane protein which contains two Ig-like C1-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. SIRPA is ubiquitously expressed. It is highly expressed in brain and detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. It is also detected on myeloid cells, but not T-cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPA acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. It may play a key role in intracellular signaling during synaptogenesis and in synaptic function. SIRPA is involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. It mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation.
Full Name
signal-regulatory protein alpha
References
  • Timms JF. et al., 1999, Curr Biol. 9: 927-30.
  • Stofega MR. et al., 2000, J Biol Chem. 275: 28222-9.
  • Liu T. et al., 2005, J Proteome Res. 4: 2070-80.
  • Wolf-Yadlin A. et al., 2007, Proc Natl Acad Sci. 104: 5860-5.

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